We created two new series of vectors specifically designed for the endogenous-locus tagging of genes encoding GPI-anchored and transmembrane domain sequences containing N-terminal signal sequences.

These vectors are called the pSiG and pSiS series, respectively, and include the incorporation of a “superfolder” fluorescent protein with improved folding dynamics and greater resistance to the change in redox environments encountered in the ER lumen or extracellular space, plus an epitope tag.

These plasmids are highly modular for easy tagging at the endogenous locus at either N- or C-terminus. They are described in Gadelha et al. 2015 Molecular and Cellular Proteomics.