LOCUS pSiG_PhsfGG 5174 bp ds-DNA circular 09-DEC-2021 DEFINITION . ACCESSION VERSION SOURCE . ORGANISM . COMMENT COMMENT COMMENT ApEinfo:methylated:1 FEATURES Location/Qualifiers rep_origin 4277..4959 /label=ColE1 origin /ApEinfo_fwdcolor=gray50 /ApEinfo_revcolor=gray50 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 sig_peptide 1222..1248 /label=HA /ApEinfo_fwdcolor=#d6394b /ApEinfo_revcolor=#d6394b /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 sig_peptide 1255..1281 /label=HA(1) /ApEinfo_label=HA /ApEinfo_fwdcolor=#d6394b /ApEinfo_revcolor=#d6394b /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 CDS 3520..4179 /label=AmpR /ApEinfo_fwdcolor=yellow /ApEinfo_revcolor=yellow /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 sig_peptide 1288..1314 /label=HA(2) /ApEinfo_label=HA /ApEinfo_fwdcolor=#d6394b /ApEinfo_revcolor=#d6394b /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 CDS 1327..2040 /label=sfGFP(-KpnI-PvuII) /ApEinfo_fwdcolor=#70fe00 /ApEinfo_revcolor=#70fe00 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 3'UTR 2149..2851 /label=Aldolase 3' /ApEinfo_fwdcolor=#bfbfbf /ApEinfo_revcolor=#bfbfbf /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 5'UTR 17..144 /label=Actin 5' /ApEinfo_fwdcolor=#bfbfbf /ApEinfo_revcolor=#bfbfbf /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 757..1107 /label=PFR2 intergenic /ApEinfo_fwdcolor=#73bed4 /ApEinfo_revcolor=#73bed4 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 sig_peptide 1120..1209 /label=VSG221 Signal Peptide /ApEinfo_fwdcolor=#fbee80 /ApEinfo_revcolor=#fbee80 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 CDS 151..747 /label=PURO /ApEinfo_fwdcolor=#0064ff /ApEinfo_revcolor=#0064ff /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 sig_peptide 2053..2139 /label=VSG221 GPI anchor /ApEinfo_fwdcolor=#fb7480 /ApEinfo_revcolor=#fb7480 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 ORIGIN 1 cagctgtacc gtcgacAGGC TAGGGCACAG CAAGGTCTTC TGAAATTCAT GTTTTTTTTT 61 TTTTTACTCT GCATTGCAGT CTCCGCTCTT ATTTAGTTTT GCTTTACGTA AGGTCTCGTT 121 GCTGCCATAA AATAAGCTCT AGTGagatcc ATGACCGAGT ACAAGCCCAC GGTGCGCCTC 181 GCCACCCGCG ACGACGTCCC CAGGGCCGTA CGCACCCTCG CCGCCGCGTT CGCCGACTAC 241 CCCGCCACGC GCCACACCGT CGATCCAGAC CGCCACATCG AGCGGGTCAC CGAGCTGCAA 301 GAACTCTTCC TCACGCGCGT CGGGCTCGAC ATCGGCAAGG TGTGGGTCGC GGACGACGGC 361 GCAGCAGTGG CGGTCTGGAC CACGCCGGAG AGCGTCGAAG CGGGGGCGGT GTTCGCCGAG 421 ATCGGCCCGC GCATGGCCGA GTTGAGCGGT TCCCGGCTGG CCGCGCAGCA ACAGATGGAA 481 GGCCTCCTGG CGCCGCACCG GCCCAAGGAG CCCGCGTGGT TCCTGGCCAC CGTCGGCGTC 541 TCGCCCGACC ACCAGGGCAA GGGTCTGGGC AGCGCCGTCG TGCTCCCCGG AGTGGAGGCG 601 GCCGAGCGCG CCGGGGTGCC CGCCTTCCTG GAGACCTCCG CGCCCCGCAA CCTCCCCTTC 661 TACGAGCGGC TCGGCTTCAC CGTCACCGCC GACGTCGAGG TGCCCGAAGG ACCGCGCACT 721 TGGTGCATGA CCCGCAAGCC CGGTGCCtaa tCTAGCcgct gcgcttaaat gtctttcatt 781 ataatcaatg tataaccttt atgtagtatt tcaatctatg ccgctgtgta cgtgcactgc 841 ggtgcctatc cttcggcatt agagagtcac tgtttgtgta gatcgtagct gcatgtctga 901 catctggttc attagtgccg ttttgagtct gcactgttgt gcaacttgat atgcatcact 961 gcacaccaac cgtgtgtttc ctcctttggg tgcacccttt aatccttgtc ttctcctttt 1021 ttgtctcttt ccccctcgaa aaggtgtcaa actactgccg cataaactac ggttatccca 1081 aatttaagag aaagcaataa agcatcaGGT ACCaagctta tgccttccaa tcaggaggcc 1141 cggcttttcc tcgccgtctt ggtcctagcc caagttcttc caattcttgt cgattcggcg 1201 gctgaaaaac ctagggccac ctacccatat gatgtgcctg actacgctgg tagctatcct 1261 tacgatgtgc cagactacgc aggaggctac ccatacgacg tgcctgatta tgccctcgag 1321 actagtatgc gtaaaggcga agagctgttc actggtgtcg tccctattct ggtggaactg 1381 gatggtgatg tcaacggtca taagttttcc gtgcgtggcg agggtgaagg tgacgcaact 1441 aatggtaaac tgacgctgaa gttcatctgt actactggta aactgccggt tccttggccg 1501 actctggtaa cgacgctgac ttatggtgtt cagtgctttg ctcgttatcc ggaccatatg 1561 aagcagcatg acttcttcaa gtccgccatg ccggaaggct atgtgcagga acgcacgatt 1621 tcctttaagg atgacggcac gtacaaaacg cgtgcggaag tgaaatttga aggcgatacc 1681 ctggtaaacc gcattgagct gaaaggcatt gactttaaag aagacggcaa tatcctgggc 1741 cataagctgg aatacaattt taacagccac aatgtttaca tcaccgccga taaacaaaaa 1801 aatggcatta aagcgaattt taaaattcgc cacaacgtgg aggatggcag cgtgcaactg 1861 gctgatcact accagcaaaa cactccaatc ggtgatggtc ctgttctgct gccagacaat 1921 cactatctga gcacgcaaag cgttctgtct aaagatccga acgagaaacg cgatcatatg 1981 gttctgctgg agttcgtaac cgcagcgggc atcacgcatg gtatggatga actgtacaaa 2041 ggaacctcta gagggaaaac tggaaacaca aacaccacag gaagcagcaa ttcttttgtc 2101 attagcaaga cccctctttg gcttgcagtt ttgctttttt aaggatccCC ATTTAGTTGG 2161 CTTTTCCCTT GTCTCGTGTC TTTTCCGTGG AAAGGTTCCC GGAGTAATCT GATGGCACAG 2221 CAGGGAGGTG CGCCTGCAGG TTGGTTAGGA AGGGGGGATG ATGTAAAAGA AGAAAATGGG 2281 GGGATATCTT ATGTTTAAGA GGATAAATAA TGTGAAGGGG CTTTATATGC TTGCTTGGTT 2341 GTCTCGTTGG TGCATGGGGA ATCTGCATGT TTGCTTTGGA GCACGCGTGG TACATCAATG 2401 GGATCATATC TTGCTGCCTC CCGCAGCTCA CCGTGCGAGC TGCCGGGACC CCGTTTCTCA 2461 TAGGCGTGTG CACCTTCCGT CTGAGACCTG TAAATGGTTA AAAAGGAATA TATAATTACC 2521 TTTTGAAAAG TGGTAAAAAA CGAATATATC TTTTTTTTTG GTTTTAATAC GCTCTTTTGT 2581 GTGTATGAGA GGAATAAATA AGTGTGTGTG TGTGTGTGTG TTGTTGTTGT TGAAAAAATG 2641 AAGGACACGA AAGCGCTCAT TAAGGGTGGG GATACACCAG GCATAATTAT CCGAAAATAT 2701 TTTGATGCAC CAAATAAGTG AACATACTGA CGAAATCAAA GAGTTGGAGG ATGGATAGGG 2761 AGGCCTCATT GGCAGTGGTA AATTGATTTG ACTTGAATAT GATACCCTCT TACTGTTTCA 2821 TTGCCACTAC AAGTTGGTTT CCTTCCCCTG CgaattcGAT GGCTTTCCCC GTCAAGCTCT 2881 AAATCGGGGG CTCCCTTTAG GGTTCCGATT TAGTGCTTTA CGGCACCTCG ACCCCAAAAA 2941 ACTTGATTAG GGTGATGGTT CACGTAGTGG GCCATCGCCC TGATAGACGG TTTTTCGCCC 3001 TTTGACGTTG GAGTCCACGT TCTTTAATAG TGGACTCTTG TTCCAAACTG GAACAACACT 3061 CAACCCTATC TCGGTCTATT CTTTTGATTT ATAAGGGATT TTGCCGATTT CGGCCTATTG 3121 GTTAAAAAAT GAGCTGATTT AACAAAAATT TAACGCGAAT TTTAACAAAA TATTAACGTT 3181 TACAATTTCC CAGGTGGCAC TTTTCGGGGA AATGTGCGCG GAACCCCTAT TTGTTTATTT 3241 TTCTAAATAC ATTCAAATAT GTATCCGCTC ATGAGACAAT AACCCTGATA AATGCTTCAA 3301 TAATATTGAA AAAGGAAGAG TATGAGTATT CAACATTTCC GTGTCGCCCT TATTCCCTTT 3361 TTTGCGGCAT TTTGCCTTCC TGTTTTTGCT CACCCAGAAA CGCTGGTGAA AGTAAAAGAT 3421 GCTGAAGATC AGTTGGGTGC ACGAGTGGGT TACATCGAAC TGGATCTCAA CAGCGGTAAG 3481 ATCCTTGAGA GTTTTCGCCC CGAAGAACGT TTTCCAATGA TGAGCACTTT TAAAGTTCTG 3541 CTATGTGGCG CGGTATTATC CCGTATTGAC GCCGGGCAAG AGCAACTCGG TCGCCGCATA 3601 CACTATTCTC AGAATGACTT GGTTGAGTAC TCACCAGTCA CAGAAAAGCA TCTTACGGAT 3661 GGCATGACAG TAAGAGAATT ATGCAGTGCT GCCATAACCA TGAGTGATAA CACTGCGGCC 3721 AACTTACTTC TGACAACGAT CGGAGGACCG AAGGAGCTAA CCGCTTTTTT GCACAACATG 3781 GGGGATCATG TAACTCGCCT TGATCGTTGG GAACCGGAGC TGAATGAAGC CATACCAAAC 3841 GACGAGCGTG ACACCACGAT GCCTGTAGCA ATGGCAACAA CGTTGCGCAA ACTATTAACT 3901 GGCGAACTAC TTACTCTAGC TTCCCGGCAA CAATTAATAG ACTGGATGGA GGCGGATAAA 3961 GTTGCAGGAC CACTTCTGCG CTCGGCCCTT CCGGCTGGCT GGTTTATTGC TGATAAATCT 4021 GGAGCCGGTG AGCGTGGGTC TCGCGGTATC ATTGCAGCAC TGGGGCCAGA TGGTAAGCCC 4081 TCCCGTATCG TAGTTATCTA CACGACGGGG AGTCAGGCAA CTATGGATGA ACGAAATAGA 4141 CAGATCGCTG AGATAGGTGC CTCACTGATT AAGCATTGGT AACTGTCAGA CCAAGTTTAC 4201 TCATATATAC TTTAGATTGA TTTAAAACTT CATTTTTAAT TTAAAAGGAT CTAGGTGAAG 4261 ATCCTTTTTG ATAATCTCAT GACCAAAATC CCTTAACGTG AGTTTTCGTT CCACTGAGCG 4321 TCAGACCCCG TAGAAAAGAT CAAAGGATCT TCTTGAGATC CTTTTTTTCT GCGCGTAATC 4381 TGCTGCTTGC AAACAAAAAA ACCACCGCTA CCAGCGGTGG TTTGTTTGCC GGATCAAGAG 4441 CTACCAACTC TTTTTCCGAA GGTAACTGGC TTCAGCAGAG CGCAGATACC AAATACTGTC 4501 CTTCTAGTGT AGCCGTAGTT AGGCCACCAC TTCAAGAACT CTGTAGCACC GCCTACATAC 4561 CTCGCTCTGC TAATCCTGTT ACCAGTGGCT GCTGCCAGTG GCGATAAGTC GTGTCTTACC 4621 GGGTTGGACT CAAGACGATA GTTACCGGAT AAGGCGCAGC GGTCGGGCTG AACGGGGGGT 4681 TCGTGCACAC AGCCCAGCTT GGAGCGAACG ACCTACACCG AACTGAGATA CCTACAGCGT 4741 GAGCTATGAG AAAGCGCCAC GCTTCCCGAA GGGAGAAAGG CGGACAGGTA TCCGGTAAGC 4801 GGCAGGGTCG GAACAGGAGA GCGCACGAGG GAGCTTCCAG GGGGAAACGC CTGGTATCTT 4861 TATAGTCCTG TCGGGTTTCG CCACCTCTGA CTTGAGCGTC GATTTTTGTG ATGCTCGTCA 4921 GGGGGGCGGA GCCTATGGAA AAACGCCAGC AACGCGGCCT TTTTACGGTT CCTGGCCTTT 4981 TGCTGGCCTT TTGCTCACAT GTTCTTTCCT GCGTTATCCC CTGATTCTGT GGATAACCGT 5041 ATTACCGCCT TTGAGTGAGC TGATACCGCT CGCCGCAGCC GAACGACCGA GCGCAGCGAG 5101 TCAGTGAGCG AGGAAGCGGA AGAGCGCCCA ATACGCAAAC CGCCTCTCCC CGCGCGTTGG 5161 CCGATTCATT AATG //