LOCUS p2T7-TcoV 7437 bp ds-DNA circular 12-AUG-2020 DEFINITION . ACCESSION VERSION SOURCE . ORGANISM . COMMENT COMMENT ApEinfo:methylated:1 FEATURES Location/Qualifiers CDS 4176..4977 /label=TcIL3000.A.H_000093600 /ApEinfo_fwdcolor=#f8766d /ApEinfo_revcolor=#f8766d CDS 643..898 /label=TcIL3000.A.H_000093600(1) /ApEinfo_label=TcIL3000.A.H_000093600 /ApEinfo_fwdcolor=#f8766d /ApEinfo_revcolor=#f8766d CDS 1407..2010 /label=eGFP fragment /ApEinfo_fwdcolor=#70fe00 /ApEinfo_revcolor=#70fe00 primer_bind 1320..1339 /label=T7 /ApEinfo_fwdcolor=#d6d4ff /ApEinfo_revcolor=#fbc0fc protein_bind complement(2054..2072) /label=TetO /ApEinfo_fwdcolor=#b6897e /ApEinfo_revcolor=#b6897e 5'UTR 110..642 /label=TcIL3000.A.H_000093600 5' /ApEinfo_fwdcolor=#ecb669 /ApEinfo_revcolor=#ecb669 rep_origin 6415..7097 /label=ColE1 origin /ApEinfo_fwdcolor=gray50 /ApEinfo_revcolor=gray50 3'UTR 3852..4134 /label=ACT 3' /ApEinfo_fwdcolor=#bfbfbf /ApEinfo_revcolor=#bfbfbf terminator 2423..2550 /label=T7 terminator /ApEinfo_fwdcolor=#937644 /ApEinfo_revcolor=#937644 terminator complement(1058..1186) /label=T7 terminator(1) /ApEinfo_label=T7 terminator /ApEinfo_fwdcolor=#937644 /ApEinfo_revcolor=#937644 CDS 5658..6317 /label=AmpR /ApEinfo_fwdcolor=#d8b6b0 /ApEinfo_revcolor=#d8b6b0 primer_bind complement(2078..2097) /label=T7(1) /ApEinfo_label=T7 /ApEinfo_fwdcolor=#d6d4ff /ApEinfo_revcolor=#fbc0fc terminator 2291..2418 /label=T7 terminator(2) /ApEinfo_label=T7 terminator /ApEinfo_fwdcolor=#937644 /ApEinfo_revcolor=#937644 terminator complement(925..1053) /label=T7 terminator(3) /ApEinfo_label=T7 terminator /ApEinfo_fwdcolor=#937644 /ApEinfo_revcolor=#937644 protein_bind 1345..1363 /label=TetO(1) /ApEinfo_label=TetO /ApEinfo_fwdcolor=#b6897e /ApEinfo_revcolor=#b6897e promoter 2595..2885 /label=rRNA promoter /ApEinfo_fwdcolor=#8986ff /ApEinfo_revcolor=#8986ff CDS 3020..3814 /label=NEO /ApEinfo_fwdcolor=#0064ff /ApEinfo_revcolor=#0064ff 5'UTR 2894..3004 /label=ACT 5' /ApEinfo_fwdcolor=#bfbfbf /ApEinfo_revcolor=#bfbfbf ORIGIN 1 GAATTGTGAG CGGATAACAA TTTCACACAG GAAACAGCTA TGACCATGAT TACGCCAAGC 61 TCGGAATTAA CCCTCACTAA AGGGAACAAA AGCTGgagct cgcggccgCa tgtgaaggta 121 cctctgtgtt taatgtacct tttgcatctg cttaaagtta atgagctagt cttcttagtc 181 tcagaaaggt gatgaaatag cattctgaca cttttaattc cactaggaac gttattatta 241 accataagag gaaagcaagc attgaaaagg gttgacttgg gctgctgaag aacccaagaa 301 ccatgctacc acaataccta agtttttaaa aagtacgtga gtgttttttt taacgaaaat 361 tccggcgcaa attttttcta tataggctcc tactagcagg agattatgga agtattcgtt 421 agttttcaca ggttccattc tagaatctaa agaatgctcc ggatttttag caggtgttta 481 actatcacca tgctcactac gtttttttct ctttgcttca aagggaaaaa ggtttcccac 541 agtccgttaa attaaattgc acccgactaa atcagagaaa caaacgccaa tcctgtacat 601 cccctccgat cccttttgga ccaactttaa taagaagtag gaatgcgtgt acatcttgca 661 attttccttg tggtcacatt gttgcggaaa ggaaccccga ccggtgaaaa cgtcaacgta 721 aacgagtatg gagttctgtg caagatatgg aggctccacc aagggcttga gttacctgac 781 ggcgagaaga ccaaagagga tggggtagca attgtgaaag aaatatcaga tttgtggata 841 gttacattgg ccaacgaatc cgaggaaaaa gattatggcg gaaaagtgga cagagagcCT 901 AGCGAATTCC TGCAGGGGCC CTATCCGGAT ATAGTTCCTC CTTTCAGCAA AAAACCCCTC 961 AAGACCCGTT TAGAGGCCCC AAGGGGTTAT GCTAGTTATT GCTCAGCGGT GGCAGCAGCC 1021 AACTCAGCTT CCTTTCGGGC TTTGTTAGCA GCCCTATCCG GATATAGTTC CTCCTTTCAG 1081 CAAAAAACCC CTCAAGACCC GTTTAGAGGC CCCAAGGGGT TATGCTAGTT ATTGCTCAGC 1141 GGTGGCAGCA GCCAACTCAG CTTCCTTTCG GGCTTTGTTA GCAGCCTGCA GCCCAGGACC 1201 GAATACTAAT AACGACACTT GCGGTCAAAA AGTAGAAGAA CAAATGCTCA ACGATGAGTG 1261 AATCAGGTTA GGGTAGTTGG AAAATTATAC AGAATGTCTT TGGCAACACA CCGCCTGATT 1321 AATACGACTC ACTATAGGGA GATCTCCCTA TCAGTGATAG AGATCTAGCC GCGGTGGCGG 1381 CCGGCCGCTC TAGAACTAGT GGATCCCACC TACGGCAAGC TGACCCTGAA GTTCATCTGC 1441 ACCACCGGCA AGCTGCCCGT GCCCTGGCCC ACCCTCGTGA CCACCCTGAC CTACGGCGTG 1501 CAGTGCTTCA GCCGCTACCC CGACCACATG AAGCAGCACG ACTTCTTCAA GTCCGCCATG 1561 CCCGAAGGCT ACGTCCAGGA GCGCACCATC TTCTTCAAGG ACGACGGCAA CTACAAGACC 1621 CGCGCCGAGG TGAAGTTCGA GGGCGACACC CTGGTGAACC GCATCGAGCT GAAGGGCATC 1681 GACTTCAAGG AGGACGGCAA CATCCTGGGG CACAAGCTGG AGTACAACTA CAACAGCCAC 1741 AACGTCTATA TCATGGCCGA CAAGCAGAAG AACGGCATCA AGGTGAACTT CAAGATCCGC 1801 CACAACATCG AGGACGGCAG CGTGCAGCTC GCCGACCACT ACCAGCAGAA CACCCCCATC 1861 GGCGACGGCC CCGTGCTGCT GCCCGACAAC CACTACCTGA GCACCCAGTC CGCCCTGAGC 1921 AAAGACCCCA ACGAGAAGCG CGATCACATG GTCCTGCTGG AGTTCGTGAC CGCCGCCGGG 1981 ATCACTCTCG GCATGGACGA GCTGTACAAG aagCTTATCG ATACCGTCGA CCTCGAGGGG 2041 GGGCCCGGGG AGATCTCTAT CACTGATAGG GAGATCTCCC TATAGTGAGT CGTATTAATC 2101 AGcCGGTGTG TTGCCAAAGA CATTCTGTAT AATTTTCCAA CTACCCTAAC CTGATTCACT 2161 CATCGTTGAG CATTTGTTCT TCTACTTTTT GACCGCAAGT GTCGTTATTA GTATTCGGTC 2221 CTCCtcgaCA TGATTGAAAT AGTGCCACCA GTTGTGTTTG ATGCGTTTGT TATCTATGCA 2281 GTATTCTGCA GGCTGCTAAC AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT GCCACCGCTG 2341 AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA 2401 AAGGAGAACT ATATCCGGAT AGGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG 2461 CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG 2521 GTTTTTTGCT GAAAGGAGAA CTATATCCGG ATtGGGCCCC TGCAGGCatg caagctagac 2581 ctagggcATG CAAGCTAGCT TTCCACCCAG CGCGGGTGCA TTCTGGCTCT TATATATACT 2641 TATTGTCATG ACAGAGTATA TTGTACTGTG TTGATAAGGG ACGGGTAACT GTATTGAAGA 2701 GCCGATGCTT TTGACATGTT AGATATAATA TGTTTTATTG TAAAGTCAAT ACAACACACA 2761 ATAGGATAAT AATGATAAAG TTAAAAAAGT ATATATAGTA ATAGAAATAT ATCTTATATA 2821 GGAAAGATTA AGCAGTAAAA GTAGCGCTTA CGGCGTACGG AGCAGGAGAG CAACTGACCG 2881 CTCTCAGACC CGGGCACAGC AAGGTCTTCT GAAATTCATG TTTTTTTTTT TTTTACTCTG 2941 CATTGCAGTC TCCGCTCTTA TTTAGTTTTG CTTTACGTAA GGTCTCGTTG CTGCCATAAA 3001 ATAAGCTATC ACCATGGttA TGATTGAACA AGATGGATTG CACGCAGGTT CTCCGGCCGC 3061 TTGGGTGGAG AGGCTATTCG GCTATGACTG GGCACAACAG ACAATCGGCT GCTCTGATGC 3121 CGCCGTGTTC CGGCTGTCAG CGCAGGGGCG CCCGGTTCTT TTTGTCAAGA CCGACCTGTC 3181 CGGTGCCCTG AATGAACTGC AGGACGAGGC AGCGCGGCTA TCGTGGCTGG CCACGACGGG 3241 CGTTCCTTGC GCAGCTGTGC TCGACGTTGT CACTGAAGCG GGAAGGGACT GGCTGCTATT 3301 GGGCGAAGTG CCGGGGCAGG ATCTCCTGTC ATCTCACCTT GCTCCTGCCG AGAAAGTATC 3361 CATCATGGCT GATGCAATGC GGCGGCTGCA TACGCTTGAT CCGGCTACCT GCCCATTCGA 3421 CCACCAAGCG AAACATCGCA TCGAGCGAGC ACGTACTCGG ATGGAAGCCG GTCTTGTCGA 3481 TCAGGATGAT CTGGACGAAG AGCATCAGGG GCTCGCGCCA GCCGAACTGT TCGCCAGGCT 3541 CAAGGCGCGC ATGCCCGACG GCGAGGATCT CGTCGTGACt CATGGCGATG CCTGCTTGCC 3601 GAATATCATG GTGGAAAATG GCCGCTTTTC TGGATTCATC GACTGTGGCC GGCTGGGTGT 3661 GGCGGACCGC TATCAGGACA TAGCGTTGGC TACCCGTGAT ATTGCTGAAG AGCTTGGCGG 3721 CGAATGGGCT GACCGCTTCC TCGTGCTTTA CGGTATCGCC GCTCCCGATT CGCAGCGCAT 3781 CGCCTTCTAT CGCCTTCTTG ACGAGTTCTT CTGACATATG TAGAGTAGAT GCCGACCGGG 3841 ATCGATCCCC CGATCCTAAC ACCGGGTTGT GTGGCCAAAA TTGTTCTGTA GTCGCTGTGA 3901 GTTGACACGG CTAGTGCTTA TGATTTTCCT CGCGTGTGGT GCCTGTACTC AGCCCTATGC 3961 CTTATTTGCA ACACATTTAC GTACAGCGCA CAAGAGAAGA GAAGATCACT TGAAGATAAT 4021 AAATATAGGG TTGTAGGCAT CTTGTTTAAC TCAAATTTTC TCGTCTTGGT GTGTCGACAT 4081 GATTGAAATA GTGCCACCAG TTGTGTTTGA TGCGTTTGTT ATCTATGCAG TATTCTGCAA 4141 GGCCTCGGAa ggagaactat atccggatag ggcCCaaaag acgcagcggt cttgggaaac 4201 gaagccgcaa aaggtgggac aatagtcaaa aattgcggtg gaaccgggga aaacgaggcc 4261 aacggcggaa atgtgggtat ttctttagcg aacgacatcc tgtgtctctg ctacctgcgt 4321 ggaagcggct acgcttccga tcagaaaatg tgcttggacg gcgtgactgg tggatccaac 4381 aataacgcct taaattctgc cctcagcgac ttaaccaatg actggccgaa attaaagaat 4441 ggatgcccca agcatcaagg aaagacgacc accgcctcca tcgaggcttc cctggccgct 4501 ttccatgcaa agctcggagc ccaggcgaac aaggctgacg ccagccacca aacagggagg 4561 tacatattgg gcattgcgac gacgccaaat acaggctgta acggggccca gaaccaattt 4621 tgcgtcaact acaaacgcca gctagcaggc accgacaaag ggatcccgtg ggagcgcaag 4681 gtccggggag ccgaagataa gctcgtggag gcggacacta tgacgcgaat ggtagaaatg 4741 ctaattcatc ggctggagaa cttgaactgg acagcttggg gaatatacga agacgcgctg 4801 gaacatgtag atgcccatga atcaggacgt atcgagggcg gctccggcac caatgccgct 4861 gccagcacct atccaaaaga aaaacaacaa aatgcaattt caaacgagag cccagtatct 4921 acttcgggat acgtaggagt gccactaggt tggttaatat cggcttcact cttccctGcg 4981 gccgctcgag aattcGATGG CTTTCCCCGT CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG 5041 TTCCGATTTA GTGCTTTACG GCACCTCGAC CCCAAAAAAC TTGATTAGGG TGATGGTTCA 5101 CGTAGTGGGC CATCGCCCTG ATAGACGGTT TTTCGCCCTT TGACGTTGGA GTCCACGTTC 5161 TTTAATAGTG GACTCTTGTT CCAAACTGGA ACAACACTCA ACCCTATCTC GGTCTATTCT 5221 TTTGATTTAT AAGGGATTTT GCCGATTTCG GCCTATTGGT TAAAAAATGA GCTGATTTAA 5281 CAAAAATTTA ACGCGAATTT TAACAAAATA TTAACGTTTA CAATTTCCCA GGTGGCACTT 5341 TTCGGGGAAA TGTGCGCGGA ACCCCTATTT GTTTATTTTT CTAAATACAT TCAAATATGT 5401 ATCCGCTCAT GAGACAATAA CCCTGATAAA TGCTTCAATA ATATTGAAAA AGGAAGAGTA 5461 TGAGTATTCA ACATTTCCGT GTCGCCCTTA TTCCCTTTTT TGCGGCATTT TGCCTTCCTG 5521 TTTTTGCTCA CCCAGAAACG CTGGTGAAAG TAAAAGATGC TGAAGATCAG TTGGGTGCAC 5581 GAGTGGGTTA CATCGAACTG GATCTCAACA GCGGTAAGAT CCTTGAGAGT TTTCGCCCCG 5641 AAGAACGTTT TCCAATGATG AGCACTTTTA AAGTTCTGCT ATGTGGCGCG GTATTATCCC 5701 GTATTGACGC CGGGCAAGAG CAACTCGGTC GCCGCATACA CTATTCTCAG AATGACTTGG 5761 TTGAGTACTC ACCAGTCACA GAAAAGCATC TTACGGATGG CATGACAGTA AGAGAATTAT 5821 GCAGTGCTGC CATAACCATG AGTGATAACA CTGCGGCCAA CTTACTTCTG ACAACGATCG 5881 GAGGACCGAA GGAGCTAACC GCTTTTTTGC ACAACATGGG GGATCATGTA ACTCGCCTTG 5941 ATCGTTGGGA ACCGGAGCTG AATGAAGCCA TACCAAACGA CGAGCGTGAC ACCACGATGC 6001 CTGTAGCAAT GGCAACAACG TTGCGCAAAC TATTAACTGG CGAACTACTT ACTCTAGCTT 6061 CCCGGCAACA ATTAATAGAC TGGATGGAGG CGGATAAAGT TGCAGGACCA CTTCTGCGCT 6121 CGGCCCTTCC GGCTGGCTGG TTTATTGCTG ATAAATCTGG AGCCGGTGAG CGTGGGTCTC 6181 GCGGTATCAT TGCAGCACTG GGGCCAGATG GTAAGCCCTC CCGTATCGTA GTTATCTACA 6241 CGACGGGGAG TCAGGCAACT ATGGATGAAC GAAATAGACA GATCGCTGAG ATAGGTGCCT 6301 CACTGATTAA GCATTGGTAA CTGTCAGACC AAGTTTACTC ATATATACTT TAGATTGATT 6361 TAAAACTTCA TTTTTAATTT AAAAGGATCT AGGTGAAGAT CCTTTTTGAT AATCTCATGA 6421 CCAAAATCCC TTAACGTGAG TTTTCGTTCC ACTGAGCGTC AGACCCCGTA GAAAAGATCA 6481 AAGGATCTTC TTGAGATCCT TTTTTTCTGC GCGTAATCTG CTGCTTGCAA ACAAAAAAAC 6541 CACCGCTACC AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT ACCAACTCTT TTTCCGAAGG 6601 TAACTGGCTT CAGCAGAGCG CAGATACCAA ATACTGTCCT TCTAGTGTAG CCGTAGTTAG 6661 GCCACCACTT CAAGAACTCT GTAGCACCGC CTACATACCT CGCTCTGCTA ATCCTGTTAC 6721 CAGTGGCTGC TGCCAGTGGC GATAAGTCGT GTCTTACCGG GTTGGACTCA AGACGATAGT 6781 TACCGGATAA GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC GTGCACACAG CCCAGCTTGG 6841 AGCGAACGAC CTACACCGAA CTGAGATACC TACAGCGTGA GCTATGAGAA AGCGCCACGC 6901 TTCCCGAAGG GAGAAAGGCG GACAGGTATC CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC 6961 GCACGAGGGA GCTTCCAGGG GGAAACGCCT GGTATCTTTA TAGTCCTGTC GGGTTTCGCC 7021 ACCTCTGACT TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC CTATGGAAAA 7081 ACGCCAGCAA CGCGGCCTTT TTACGGTTCC TGGCCTTTTG CTGGCCTTTT GCTCACATGT 7141 TCTTTCCTGC GTTATCCCCT GATTCTGTGG ATAACCGTAT TACCGCCTTT GAGTGAGCTG 7201 ATACCGCTCG CCGCAGCCGA ACGACCGAGC GCAGCGAGTC AGTGAGCGAG GAAGCGGAAG 7261 AGCGCCCAAT ACGCAAACCG CCTCTCCCCG CGCGTTGGCC GATTCATTAA TGCAGCTGGC 7321 ACGACAGGTT TCCCGACTGG AAAGCGGGCA GTGAGCGCAA CGCAATTAAT GTGAGTTAGC 7381 TCACTCATTA GGCACCCCAG GCTTTACACT TTATGCTTCC GGCTCGTATG TTGTGTG //