LOCUS pSiS_PhsfGG 5098 bp ds-DNA circular 09-DEC-2021 DEFINITION . ACCESSION VERSION SOURCE . ORGANISM . COMMENT COMMENT COMMENT ApEinfo:methylated:1 FEATURES Location/Qualifiers rep_origin 4201..4883 /label=ColE1 origin /ApEinfo_fwdcolor=gray50 /ApEinfo_revcolor=gray50 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 sig_peptide 1222..1248 /label=HA /ApEinfo_fwdcolor=#d6394b /ApEinfo_revcolor=#d6394b /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 CDS 3444..4103 /label=AmpR /ApEinfo_fwdcolor=yellow /ApEinfo_revcolor=yellow /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 sig_peptide 1255..1281 /label=HA(1) /ApEinfo_label=HA /ApEinfo_fwdcolor=#d6394b /ApEinfo_revcolor=#d6394b /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 sig_peptide 1288..1314 /label=HA(2) /ApEinfo_label=HA /ApEinfo_fwdcolor=#d6394b /ApEinfo_revcolor=#d6394b /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 CDS 1327..2040 /label=sfGFP(-KpnI-PvuII) /ApEinfo_fwdcolor=#70fe00 /ApEinfo_revcolor=#70fe00 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 3'UTR 2073..2775 /label=Aldolase 3' /ApEinfo_fwdcolor=#bfbfbf /ApEinfo_revcolor=#bfbfbf /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 5'UTR 17..144 /label=Actin 5' /ApEinfo_fwdcolor=#bfbfbf /ApEinfo_revcolor=#bfbfbf /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 757..1107 /label=PFR2 intergenic /ApEinfo_fwdcolor=#73bed4 /ApEinfo_revcolor=#73bed4 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 sig_peptide 1120..1209 /label=VSG221 Signal Peptide /ApEinfo_fwdcolor=#fbee80 /ApEinfo_revcolor=#fbee80 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 CDS 151..747 /label=PURO /ApEinfo_fwdcolor=#0064ff /ApEinfo_revcolor=#0064ff /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 ORIGIN 1 cagctgtacc gtcgacAGGC TAGGGCACAG CAAGGTCTTC TGAAATTCAT GTTTTTTTTT 61 TTTTTACTCT GCATTGCAGT CTCCGCTCTT ATTTAGTTTT GCTTTACGTA AGGTCTCGTT 121 GCTGCCATAA AATAAGCTCT AGTGagatcc ATGACCGAGT ACAAGCCCAC GGTGCGCCTC 181 GCCACCCGCG ACGACGTCCC CAGGGCCGTA CGCACCCTCG CCGCCGCGTT CGCCGACTAC 241 CCCGCCACGC GCCACACCGT CGATCCAGAC CGCCACATCG AGCGGGTCAC CGAGCTGCAA 301 GAACTCTTCC TCACGCGCGT CGGGCTCGAC ATCGGCAAGG TGTGGGTCGC GGACGACGGC 361 GCAGCAGTGG CGGTCTGGAC CACGCCGGAG AGCGTCGAAG CGGGGGCGGT GTTCGCCGAG 421 ATCGGCCCGC GCATGGCCGA GTTGAGCGGT TCCCGGCTGG CCGCGCAGCA ACAGATGGAA 481 GGCCTCCTGG CGCCGCACCG GCCCAAGGAG CCCGCGTGGT TCCTGGCCAC CGTCGGCGTC 541 TCGCCCGACC ACCAGGGCAA GGGTCTGGGC AGCGCCGTCG TGCTCCCCGG AGTGGAGGCG 601 GCCGAGCGCG CCGGGGTGCC CGCCTTCCTG GAGACCTCCG CGCCCCGCAA CCTCCCCTTC 661 TACGAGCGGC TCGGCTTCAC CGTCACCGCC GACGTCGAGG TGCCCGAAGG ACCGCGCACT 721 TGGTGCATGA CCCGCAAGCC CGGTGCCtaa tCTAGCcgct gcgcttaaat gtctttcatt 781 ataatcaatg tataaccttt atgtagtatt tcaatctatg ccgctgtgta cgtgcactgc 841 ggtgcctatc cttcggcatt agagagtcac tgtttgtgta gatcgtagct gcatgtctga 901 catctggttc attagtgccg ttttgagtct gcactgttgt gcaacttgat atgcatcact 961 gcacaccaac cgtgtgtttc ctcctttggg tgcacccttt aatccttgtc ttctcctttt 1021 ttgtctcttt ccccctcgaa aaggtgtcaa actactgccg cataaactac ggttatccca 1081 aatttaagag aaagcaataa agcatcaGGT ACCaagctta tgccttccaa tcaggaggcc 1141 cggcttttcc tcgccgtctt ggtcctagcc caagttcttc caattcttgt cgattcggcg 1201 gctgaaaaac ctagggccac ctacccatat gatgtgcctg actacgctgg tagctatcct 1261 tacgatgtgc cagactacgc aggaggctac ccatacgacg tgcctgatta tgccctcgag 1321 actagtatgc gtaaaggcga agagctgttc actggtgtcg tccctattct ggtggaactg 1381 gatggtgatg tcaacggtca taagttttcc gtgcgtggcg agggtgaagg tgacgcaact 1441 aatggtaaac tgacgctgaa gttcatctgt actactggta aactgccggt tccttggccg 1501 actctggtaa cgacgctgac ttatggtgtt cagtgctttg ctcgttatcc ggaccatatg 1561 aagcagcatg acttcttcaa gtccgccatg ccggaaggct atgtgcagga acgcacgatt 1621 tcctttaagg atgacggcac gtacaaaacg cgtgcggaag tgaaatttga aggcgatacc 1681 ctggtaaacc gcattgagct gaaaggcatt gactttaaag aagacggcaa tatcctgggc 1741 cataagctgg aatacaattt taacagccac aatgtttaca tcaccgccga taaacaaaaa 1801 aatggcatta aagcgaattt taaaattcgc cacaacgtgg aggatggcag cgtgcaactg 1861 gctgatcact accagcaaaa cactccaatc ggtgatggtc ctgttctgct gccagacaat 1921 cactatctga gcacgcaaag cgttctgtct aaagatccga acgagaaacg cgatcatatg 1981 gttctgctgg agttcgtaac cgcagcgggc atcacgcatg gtatggatga actgtacaaa 2041 ggaacctcta gatagatcac tggaaaggat ccCCATTTAG TTGGCTTTTC CCTTGTCTCG 2101 TGTCTTTTCC GTGGAAAGGT TCCCGGAGTA ATCTGATGGC ACAGCAGGGA GGTGCGCCTG 2161 CAGGTTGGTT AGGAAGGGGG GATGATGTAA AAGAAGAAAA TGGGGGGATA TCTTATGTTT 2221 AAGAGGATAA ATAATGTGAA GGGGCTTTAT ATGCTTGCTT GGTTGTCTCG TTGGTGCATG 2281 GGGAATCTGC ATGTTTGCTT TGGAGCACGC GTGGTACATC AATGGGATCA TATCTTGCTG 2341 CCTCCCGCAG CTCACCGTGC GAGCTGCCGG GACCCCGTTT CTCATAGGCG TGTGCACCTT 2401 CCGTCTGAGA CCTGTAAATG GTTAAAAAGG AATATATAAT TACCTTTTGA AAAGTGGTAA 2461 AAAACGAATA TATCTTTTTT TTTGGTTTTA ATACGCTCTT TTGTGTGTAT GAGAGGAATA 2521 AATAAGTGTG TGTGTGTGTG TGTGTTGTTG TTGTTGAAAA AATGAAGGAC ACGAAAGCGC 2581 TCATTAAGGG TGGGGATACA CCAGGCATAA TTATCCGAAA ATATTTTGAT GCACCAAATA 2641 AGTGAACATA CTGACGAAAT CAAAGAGTTG GAGGATGGAT AGGGAGGCCT CATTGGCAGT 2701 GGTAAATTGA TTTGACTTGA ATATGATACC CTCTTACTGT TTCATTGCCA CTACAAGTTG 2761 GTTTCCTTCC CCTGCgaatt cGATGGCTTT CCCCGTCAAG CTCTAAATCG GGGGCTCCCT 2821 TTAGGGTTCC GATTTAGTGC TTTACGGCAC CTCGACCCCA AAAAACTTGA TTAGGGTGAT 2881 GGTTCACGTA GTGGGCCATC GCCCTGATAG ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC 2941 ACGTTCTTTA ATAGTGGACT CTTGTTCCAA ACTGGAACAA CACTCAACCC TATCTCGGTC 3001 TATTCTTTTG ATTTATAAGG GATTTTGCCG ATTTCGGCCT ATTGGTTAAA AAATGAGCTG 3061 ATTTAACAAA AATTTAACGC GAATTTTAAC AAAATATTAA CGTTTACAAT TTCCCAGGTG 3121 GCACTTTTCG GGGAAATGTG CGCGGAACCC CTATTTGTTT ATTTTTCTAA ATACATTCAA 3181 ATATGTATCC GCTCATGAGA CAATAACCCT GATAAATGCT TCAATAATAT TGAAAAAGGA 3241 AGAGTATGAG TATTCAACAT TTCCGTGTCG CCCTTATTCC CTTTTTTGCG GCATTTTGCC 3301 TTCCTGTTTT TGCTCACCCA GAAACGCTGG TGAAAGTAAA AGATGCTGAA GATCAGTTGG 3361 GTGCACGAGT GGGTTACATC GAACTGGATC TCAACAGCGG TAAGATCCTT GAGAGTTTTC 3421 GCCCCGAAGA ACGTTTTCCA ATGATGAGCA CTTTTAAAGT TCTGCTATGT GGCGCGGTAT 3481 TATCCCGTAT TGACGCCGGG CAAGAGCAAC TCGGTCGCCG CATACACTAT TCTCAGAATG 3541 ACTTGGTTGA GTACTCACCA GTCACAGAAA AGCATCTTAC GGATGGCATG ACAGTAAGAG 3601 AATTATGCAG TGCTGCCATA ACCATGAGTG ATAACACTGC GGCCAACTTA CTTCTGACAA 3661 CGATCGGAGG ACCGAAGGAG CTAACCGCTT TTTTGCACAA CATGGGGGAT CATGTAACTC 3721 GCCTTGATCG TTGGGAACCG GAGCTGAATG AAGCCATACC AAACGACGAG CGTGACACCA 3781 CGATGCCTGT AGCAATGGCA ACAACGTTGC GCAAACTATT AACTGGCGAA CTACTTACTC 3841 TAGCTTCCCG GCAACAATTA ATAGACTGGA TGGAGGCGGA TAAAGTTGCA GGACCACTTC 3901 TGCGCTCGGC CCTTCCGGCT GGCTGGTTTA TTGCTGATAA ATCTGGAGCC GGTGAGCGTG 3961 GGTCTCGCGG TATCATTGCA GCACTGGGGC CAGATGGTAA GCCCTCCCGT ATCGTAGTTA 4021 TCTACACGAC GGGGAGTCAG GCAACTATGG ATGAACGAAA TAGACAGATC GCTGAGATAG 4081 GTGCCTCACT GATTAAGCAT TGGTAACTGT CAGACCAAGT TTACTCATAT ATACTTTAGA 4141 TTGATTTAAA ACTTCATTTT TAATTTAAAA GGATCTAGGT GAAGATCCTT TTTGATAATC 4201 TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA 4261 AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA 4321 AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC 4381 CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTCCTTCTA GTGTAGCCGT 4441 AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC 4501 TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC 4561 GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA 4621 GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG 4681 CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG 4741 GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT 4801 TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT 4861 GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC 4921 ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTATTACC GCCTTTGAGT 4981 GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG 5041 CGGAAGAGCG CCCAATACGC AAACCGCCTC TCCCCGCGCG TTGGCCGATT CATTAATG //