LOCUS pTcoSce_tiT7 7953 bp ds-DNA circular 13-AUG-2020 DEFINITION . ACCESSION VERSION SOURCE . ORGANISM . COMMENT COMMENT ApEinfo:methylated:1 FEATURES Location/Qualifiers CDS 2783..3805 /label=HYG /ApEinfo_fwdcolor=#0064ff /ApEinfo_revcolor=#0064ff CDS 4692..5493 /label=TcIL3000.A.H_000093600 /ApEinfo_fwdcolor=#f8766d /ApEinfo_revcolor=#f8766d CDS 643..897 /label=TcIL3000.A.H_000093600(1) /ApEinfo_label=TcIL3000.A.H_000093600 /ApEinfo_fwdcolor=#f8766d /ApEinfo_revcolor=#f8766d 3'UTR 3837..4119 /label=ACT 3' /ApEinfo_fwdcolor=#bfbfbf /ApEinfo_revcolor=#bfbfbf 5'UTR 110..642 /label=TcIL3000.A.H_000093600 5' /ApEinfo_fwdcolor=#ecb669 /ApEinfo_revcolor=#ecb669 3'UTR 4135..4417 /label=ACT 3'(1) /ApEinfo_label=ACT 3' /ApEinfo_fwdcolor=#bfbfbf /ApEinfo_revcolor=#bfbfbf rep_origin 6931..7613 /label=ColE1 origin /ApEinfo_fwdcolor=gray50 /ApEinfo_revcolor=gray50 primer_bind 2621..2640 /label=T7 /ApEinfo_fwdcolor=#d6d4ff /ApEinfo_revcolor=#fbc0fc CDS 6174..6833 /label=AmpR /ApEinfo_fwdcolor=#d8b6b0 /ApEinfo_revcolor=#d8b6b0 protein_bind 950..968 /label=TetO /ApEinfo_fwdcolor=#b6897e /ApEinfo_revcolor=#b6897e 3'UTR 1884..2593 /label=ALD 3' /ApEinfo_fwdcolor=#bfbfbf /ApEinfo_revcolor=#bfbfbf protein_bind 971..989 /label=TetO(1) /ApEinfo_label=TetO /ApEinfo_fwdcolor=#b6897e /ApEinfo_revcolor=#b6897e 5'UTR 1022..1114 /label=EP1 5' /ApEinfo_fwdcolor=#bfbfbf /ApEinfo_revcolor=#bfbfbf misc_feature 926..945 /label=T7(1) /ApEinfo_label=T7 /ApEinfo_fwdcolor=#d6d4ff /ApEinfo_revcolor=#fbc0fc terminator 4423..4550 /label=T7 terminator /ApEinfo_fwdcolor=#937644 /ApEinfo_revcolor=#937644 5'UTR 2659..2767 /label=ACT 5' /ApEinfo_fwdcolor=#bfbfbf /ApEinfo_revcolor=#bfbfbf terminator 4555..4682 /label=T7 terminator(1) /ApEinfo_label=T7 terminator /ApEinfo_fwdcolor=#937644 /ApEinfo_revcolor=#937644 misc_feature 904..921 /label=I-SceI_recognition_seq /ApEinfo_fwdcolor=#bb48ff /ApEinfo_revcolor=#bb48ff CDS 1116..1880 /label=NLS-SceI-HA /ApEinfo_fwdcolor=#aa0000 /ApEinfo_revcolor=#aa0000 ORIGIN 1 GAATTGTGAG CGGATAACAA TTTCACACAG GAAACAGCTA TGACCATGAT TACGCCAAGC 61 TCGGAATTAA CCCTCACTAA AGGGAACAAA AGCTGgagct cgcggccgCa tgtgaaggta 121 cctctgtgtt taatgtacct tttgcatctg cttaaagtta atgagctagt cttcttagtc 181 tcagaaaggt gatgaaatag cattctgaca cttttaattc cactaggaac gttattatta 241 accataagag gaaagcaagc attgaaaagg gttgacttgg gctgctgaag aacccaagaa 301 ccatgctacc acaataccta agtttttaaa aagtacgtga gtgttttttt taacgaaaat 361 tccggcgcaa attttttcta tataggctcc tactagcagg agattatgga agtattcgtt 421 agttttcaca ggttccattc tagaatctaa agaatgctcc ggatttttag caggtgttta 481 actatcacca tgctcactac gtttttttct ctttgcttca aagggaaaaa ggtttcccac 541 agtccgttaa attaaattgc acccgactaa atcagagaaa caaacgccaa tcctgtacat 601 cccctccgat cccttttgga ccaactttaa taagaagtag gaatgcgtgt acatcttgca 661 attttccttg tggtcacatt gttgcggaaa ggaaccccga ccggtgaaaa cgtcaacgta 721 aacgagtatg gagttctgtg caagatatgg aggctccacc aagggcttga gttacctgac 781 ggcgagaaga ccaaagagga tggggtagca attgtgaaag aaatatcaga tttgtggata 841 gttacattgg ccaacgaatc cgaggaaaaa gattatggcg gaaaagtgga cagagagCCT 901 AGGtagggat aacagggtaa tgtacTAATA CGACTCACTA TAGGGCGGTT CCCTATCAGT 961 GATAGAGATC TCCCTATCAG TGATAGAGAT CCCTGAGTAC TGAGTTTAAC ATGTTCTCGT 1021 Cccgggctgc acgcgccttc gagttttttt tccttttccc catttttttc aacttgaaga 1081 cttcaattac accaaaaagt aaaattcaca agcttatgcc aaagaagaag cgaaaggtac 1141 atatgaaaaa catcaaaaaa aaccaggtaa tgaacctggg tccgaactct aaactgctga 1201 aagaatacaa atcccagctg atcgaactga acatcgaaca gttcgaagca ggtatcggtc 1261 tgatcctggg tgatgcttac atccgttctc gtgatgaagg taaaacctac tgtatgcagt 1321 tcgagtggaa aaacaaagca tacatggacc acgtatgtct gctgtacgat cagtgggtac 1381 tgtccccgcc gcacaaaaaa gaacgtgtta accacctggg taacctggta atcacctggg 1441 gcgcccagac tttcaaacac caagcattca acaaactggc taacctgttc atcgttaaca 1501 acaaaaaaac catcccgaac aacctggttg aaaactacct gaccccgatg tctctggcat 1561 actggttcat ggatgatggt ggtaaatggg attacaacaa aaactctacc aacaaatcga 1621 tcgtactgaa cacccagtct ttcactttcg aagaagtaga atacctggtt aagggtctgc 1681 gtaacaaatt ccaactgaac tgttacgtaa aaatcaacaa aaacaaaccg atcatctaca 1741 tcgattctat gtcttacctg atcttctaca acctgatcaa accgtacctg atcccgcaga 1801 tgatgtacaa actgccgaac actatctcct ccgaaacttt cctgaaatac ccatacgatg 1861 ttccagatta cgcttgctaa tagGATCCTG CCCATTTAGT TGGCTTTTCC CTTGTCTCGT 1921 GTCTTTTCCG TGGAAAGGTT CCCGGAGTAA TCTGATGGCA CAGCAGGGAG GTGCGCCTGC 1981 AGGTTGGTTA GGAAGGGGGG ATGATGTAAA AGAAGAAAAT GGGGGGATAT CTTATGTTTA 2041 AGAGGATAAA TAATGTGAAG GGGCTTTATA TGCTTGCTTG GTTGTCTCGT TGGTGCATGG 2101 GGAATCTGCA TGTTTGCTTT GGAGCACGCG TGGTACATCA ATGGGATCAT ATCTTGCTGC 2161 CTCCCGCAGT CACCGTGCGG AGCTGCCGGT GCCCCGTTTC TCATAGGCGT GTGCACCTTC 2221 CGTCTGAGAC CTGTAAATGG TTAAAAAGGA ATATATAATT ACCTTTTGAA AAGTGGTAAA 2281 AAACGAATAT ATGTTTTTTT TTGGTTTTAA TACGCTCTTT TGTGTGTATG AGAGGAATAA 2341 ATAAGTGTGT GTGTGTGTGT GTTGTTGTTG TTGAAAAAAT GAAGGACACG AAAGCGCTCA 2401 TTAGGGGTGG GGATACACCA GGCATAATTA TCCGAAAATA TTTTGGTGCA CCAAATAAGT 2461 GAACATACTG ACGAAATCAA AGAGTTGGAG GATGGATAGG GAGGCCTCAT TGGCAGTGGT 2521 AAATTGATTT GACTTGAATA TGATACCCTC TTACTGTTTC ATTGCCACTA CAAGTTGGTT 2581 TCCTTCCCCT GCAGGCATGC TGGAAAGCTA GGTACCTGAT TAATACGACT CACTATAGGG 2641 AGATCTCCCG TACCGGGGGC ACAGCAAGGT CTTCTGAAAT TCATGTTTTT TTTTTTTTAC 2701 TCTGCATTGC AGTCTCCGCT CTTATTAGTT TTGCTTTACG TAAGGTCTCG TTGCTGCCAT 2761 AAAATAAGCT CTAGAACTAG TGATGAAAAA GCCTGAACTC ACCGCGACGT CTGTCGAGAA 2821 GTTTCTGATC GAAAAGTTCG ACAGCGTCTC CGACCTGATG CAGCTCTCGG AGGGCGAAGA 2881 ATCTCGTGCT TTCAGCTTCG ATGTAGGAGG GCGTGGATAT GTCCTGCGGG TAAATAGCTG 2941 CGCCGATGGT TTCTACAAAG ATCGTTATGT TTATCGGCAC TTTGCATCGG CCGCGCTCCC 3001 GATTCCGGAA GTGCTTGACA TTGGGGAATT CAGCGAGAGC CTGACCTATT GCATCTCCCG 3061 CCGTGCACAG GGTGTCACGT TGCAAGACCT GCCTGAAACC GAACTGCCCG CTGTTCTGCA 3121 GCCGGTCGCG GAGGCCATGG ATGCGATCGC TGCGGCCGAT CTTAGCCAGA CGAGCGGGTT 3181 CGGCCCATTC GGACCGCAAG GAATCGGTCA ATACACTACA TGGCGTGATT TCATATGCGC 3241 GATTGCTGAT CCCCATGTGT ATCACTGGCA AACTGTGATG GACGACACCG TCAGTGCGTC 3301 CGTCGCGCAG GCTCTCGATG AGCTGATGCT TTGGGCCGAG GACTGCCCCG AAGTCCGGCA 3361 CCTCGTGCAC GCGGATTTCG GCTCCAACAA TGTCCTGACG GACAATGGCC GCATAACAGC 3421 GGTCATTGAC TGGAGCGAGG CGATGTTCGG GGATTCCCAA TACGAGGTCG CCAACATCTT 3481 CTTCTGGAGG CCGTGGTTGG CTTGTATGGA GCAGCAGACG CGCTACTTCG AGCGGAGGCA 3541 TCCGGAGCTT GCAGGATCGC CGCGGCTCCG GGCGTATATG CTCCGCATTG GTCTTGACCA 3601 ACTCTATCAG AGCTTGGTTG ACGGCAATTT CGATGATGCA GCTTGGGCGC AGGGTCGATG 3661 CGACGCAATC GTCCGATCCG GAGCCGGGAC TGTCGGGCGT ACACAAATCG CCCGCAGAAG 3721 CGCGGCCGTC TGGACCGATG GCTGTGTAGA AGTACTCGCC GATAGTGGAA ACCGACGCCC 3781 CAGCACTCGT CCGAGGGCAA AGGAATAGAG TAGATGCCGA CCGGGATcga tccccCGATC 3841 CTAACACCGG GTTGTGTGGC CAAAATTGTT CTGTAGTCGC TGTGAGTTGA CACGGCTAGT 3901 GCTTATGATT TTCCTCGCGT GTGGTGCCTG TACTCAGCCC TATGCCTTAT TTGCAACACA 3961 TTTACGTACA GCGCACAAGA GAAGAGAAGA TCACTTGAAG ATAATAAATA TAGGGTTGTA 4021 GGCATCTTGT TTAACTCAAA TTTTCTCGTC TTGGTGTGTC GACATGATTG AAATAGTGCC 4081 ACCAGTTGTG TTTGATGCGT TTGTTATCTA TGCAGTATTC TGCAAGGCCT CGGAGATCCT 4141 AACACCGGGT TGTGTGGCCA AAATTGTTCT GTAGTCGCTG TGAGTTGACA CGGCTAGTGC 4201 TTATGATTTT CCTCGCGTGT GGTGCCTGTA CTCAGCCCTA TGCCTTATTT GCAACACATT 4261 TACGTACAGC GCACAAGAGA AGAGAAGATC ACTTGAAGAT AATAAATATA GGGTTGTAGG 4321 CATCTTGTTT AACTCAAATT TTCTCGTCTT GGTGTGTCGA CATGATTGAA ATAGTGCCAC 4381 CAGTTGTGTT TGATGCGTTT GTTATCTATG CAGTATTCTG CAGGCTGCTA ACAAAGCCCG 4441 AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC 4501 CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGAA CTATATCCGG ATAGGGCTGC 4561 TAACAAAGCC CGAAAGGAAG CTGAGTTGGC TGCTGCCACC GCTGAGCAAT AACTAGCATA 4621 ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CTGAAAGGAG AACTATATCC 4681 GGATagggcC Caaaagacgc agcggtcttg ggaaacgaag ccgcaaaagg tgggacaata 4741 gtcaaaaatt gcggtggaac cggggaaaac gaggccaacg gcggaaatgt gggtatttct 4801 ttagcgaacg acatcctgtg tctctgctac ctgcgtggaa gcggctacgc ttccgatcag 4861 aaaatgtgct tggacggcgt gactggtgga tccaacaata acgccttaaa ttctgccctc 4921 agcgacttaa ccaatgactg gccgaaatta aagaatggat gccccaagca tcaaggaaag 4981 acgaccaccg cctccatcga ggcttccctg gccgctttcc atgcaaagct cggagcccag 5041 gcgaacaagg ctgacgccag ccaccaaaca gggaggtaca tattgggcat tgcgacgacg 5101 ccaaatacag gctgtaacgg ggcccagaac caattttgcg tcaactacaa acgccagcta 5161 gcaggcaccg acaaagggat cccgtgggag cgcaaggtcc ggggagccga agataagctc 5221 gtggaggcgg acactatgac gcgaatggta gaaatgctaa ttcatcggct ggagaacttg 5281 aactggacag cttggggaat atacgaagac gcgctggaac atgtagatgc ccatgaatca 5341 ggacgtatcg agggcggctc cggcaccaat gccgctgcca gcacctatcc aaaagaaaaa 5401 caacaaaatg caatttcaaa cgagagccca gtatctactt cgggatacgt aggagtgcca 5461 ctaggttggt taatatcggc ttcactcttc cctGcggccg ctcgagaatt cGATGGCTTT 5521 CCCCGTCAAG CTCTAAATCG GGGGCTCCCT TTAGGGTTCC GATTTAGTGC TTTACGGCAC 5581 CTCGACCCCA AAAAACTTGA TTAGGGTGAT GGTTCACGTA GTGGGCCATC GCCCTGATAG 5641 ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA ATAGTGGACT CTTGTTCCAA 5701 ACTGGAACAA CACTCAACCC TATCTCGGTC TATTCTTTTG ATTTATAAGG GATTTTGCCG 5761 ATTTCGGCCT ATTGGTTAAA AAATGAGCTG ATTTAACAAA AATTTAACGC GAATTTTAAC 5821 AAAATATTAA CGTTTACAAT TTCCCAGGTG GCACTTTTCG GGGAAATGTG CGCGGAACCC 5881 CTATTTGTTT ATTTTTCTAA ATACATTCAA ATATGTATCC GCTCATGAGA CAATAACCCT 5941 GATAAATGCT TCAATAATAT TGAAAAAGGA AGAGTATGAG TATTCAACAT TTCCGTGTCG 6001 CCCTTATTCC CTTTTTTGCG GCATTTTGCC TTCCTGTTTT TGCTCACCCA GAAACGCTGG 6061 TGAAAGTAAA AGATGCTGAA GATCAGTTGG GTGCACGAGT GGGTTACATC GAACTGGATC 6121 TCAACAGCGG TAAGATCCTT GAGAGTTTTC GCCCCGAAGA ACGTTTTCCA ATGATGAGCA 6181 CTTTTAAAGT TCTGCTATGT GGCGCGGTAT TATCCCGTAT TGACGCCGGG CAAGAGCAAC 6241 TCGGTCGCCG CATACACTAT TCTCAGAATG ACTTGGTTGA GTACTCACCA GTCACAGAAA 6301 AGCATCTTAC GGATGGCATG ACAGTAAGAG AATTATGCAG TGCTGCCATA ACCATGAGTG 6361 ATAACACTGC GGCCAACTTA CTTCTGACAA CGATCGGAGG ACCGAAGGAG CTAACCGCTT 6421 TTTTGCACAA CATGGGGGAT CATGTAACTC GCCTTGATCG TTGGGAACCG GAGCTGAATG 6481 AAGCCATACC AAACGACGAG CGTGACACCA CGATGCCTGT AGCAATGGCA ACAACGTTGC 6541 GCAAACTATT AACTGGCGAA CTACTTACTC TAGCTTCCCG GCAACAATTA ATAGACTGGA 6601 TGGAGGCGGA TAAAGTTGCA GGACCACTTC TGCGCTCGGC CCTTCCGGCT GGCTGGTTTA 6661 TTGCTGATAA ATCTGGAGCC GGTGAGCGTG GGTCTCGCGG TATCATTGCA GCACTGGGGC 6721 CAGATGGTAA GCCCTCCCGT ATCGTAGTTA TCTACACGAC GGGGAGTCAG GCAACTATGG 6781 ATGAACGAAA TAGACAGATC GCTGAGATAG GTGCCTCACT GATTAAGCAT TGGTAACTGT 6841 CAGACCAAGT TTACTCATAT ATACTTTAGA TTGATTTAAA ACTTCATTTT TAATTTAAAA 6901 GGATCTAGGT GAAGATCCTT TTTGATAATC TCATGACCAA AATCCCTTAA CGTGAGTTTT 6961 CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT 7021 TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT 7081 TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA 7141 TACCAAATAC TGTCCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG 7201 CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA 7261 AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG 7321 GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA 7381 GATACCTACA GCGTGAGCTA TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA 7441 GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA 7501 ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT 7561 TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC 7621 GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT 7681 CTGTGGATAA CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA 7741 CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG CCCAATACGC AAACCGCCTC 7801 TCCCCGCGCG TTGGCCGATT CATTAATGCA GCTGGCACGA CAGGTTTCCC GACTGGAAAG 7861 CGGGCAGTGA GCGCAACGCA ATTAATGTGA GTTAGCTCAC TCATTAGGCA CCCCAGGCTT 7921 TACACTTTAT GCTTCCGGCT CGTATGTTGT GTG //