LOCUS pTcoSce_TbrRNA 8196 bp ds-DNA circular 13-AUG-2020 DEFINITION . ACCESSION VERSION SOURCE . ORGANISM . COMMENT COMMENT ApEinfo:methylated:1 FEATURES Location/Qualifiers CDS 3026..4048 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 /label=HYG /ApEinfo_fwdcolor=#0064ff /ApEinfo_revcolor=#0064ff CDS 4935..5736 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 /label=TcIL3000.A.H_000093600 /ApEinfo_fwdcolor=#f8766d /ApEinfo_revcolor=#f8766d CDS 643..897 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 /label=TcIL3000.A.H_000093600(1) /ApEinfo_label=TcIL3000.A.H_000093600 /ApEinfo_fwdcolor=#f8766d /ApEinfo_revcolor=#f8766d 3'UTR 4080..4362 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 /label=ACT 3' /ApEinfo_fwdcolor=#bfbfbf /ApEinfo_revcolor=#bfbfbf 5'UTR 110..642 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 /label=TcIL3000.A.H_000093600 5' /ApEinfo_fwdcolor=#ecb669 /ApEinfo_revcolor=#ecb669 3'UTR 4378..4660 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 /label=ACT 3'(1) /ApEinfo_label=ACT 3' /ApEinfo_fwdcolor=#bfbfbf /ApEinfo_revcolor=#bfbfbf rep_origin 7174..7856 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 /label=ColE1 origin /ApEinfo_fwdcolor=gray50 /ApEinfo_revcolor=gray50 primer_bind 2864..2883 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 /label=T7 /ApEinfo_fwdcolor=#d6d4ff /ApEinfo_revcolor=#fbc0fc promoter 926..1188 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 /label=rDNA promoter /ApEinfo_fwdcolor=#91a2c6 /ApEinfo_revcolor=#91a2c6 CDS 6417..7076 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 /label=AmpR /ApEinfo_fwdcolor=#d8b6b0 /ApEinfo_revcolor=#d8b6b0 protein_bind 1193..1211 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 /label=TetO /ApEinfo_fwdcolor=#b6897e /ApEinfo_revcolor=#b6897e 3'UTR 2127..2836 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 /label=ALD 3' /ApEinfo_fwdcolor=#bfbfbf /ApEinfo_revcolor=#bfbfbf protein_bind 1214..1232 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 /label=TetO(1) /ApEinfo_label=TetO /ApEinfo_fwdcolor=#b6897e /ApEinfo_revcolor=#b6897e 5'UTR 1265..1357 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 /label=EP1 5' /ApEinfo_fwdcolor=#bfbfbf /ApEinfo_revcolor=#bfbfbf terminator 4666..4793 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 /label=T7 terminator /ApEinfo_fwdcolor=#937644 /ApEinfo_revcolor=#937644 5'UTR 2902..3010 /label=ACT 5' /ApEinfo_fwdcolor=#bfbfbf /ApEinfo_revcolor=#bfbfbf terminator 4798..4925 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 /label=T7 terminator(1) /ApEinfo_label=T7 terminator /ApEinfo_fwdcolor=#937644 /ApEinfo_revcolor=#937644 misc_feature 904..921 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 /label=I-SceI_recognition_seq /ApEinfo_fwdcolor=#bb48ff /ApEinfo_revcolor=#bb48ff CDS 1359..2123 /label=NLS-SceI-HA /ApEinfo_fwdcolor=#aa0000 /ApEinfo_revcolor=#aa0000 ORIGIN 1 GAATTGTGAG CGGATAACAA TTTCACACAG GAAACAGCTA TGACCATGAT TACGCCAAGC 61 TCGGAATTAA CCCTCACTAA AGGGAACAAA AGCTGgagct cgcggccgCa tgtgaaggta 121 cctctgtgtt taatgtacct tttgcatctg cttaaagtta atgagctagt cttcttagtc 181 tcagaaaggt gatgaaatag cattctgaca cttttaattc cactaggaac gttattatta 241 accataagag gaaagcaagc attgaaaagg gttgacttgg gctgctgaag aacccaagaa 301 ccatgctacc acaataccta agtttttaaa aagtacgtga gtgttttttt taacgaaaat 361 tccggcgcaa attttttcta tataggctcc tactagcagg agattatgga agtattcgtt 421 agttttcaca ggttccattc tagaatctaa agaatgctcc ggatttttag caggtgttta 481 actatcacca tgctcactac gtttttttct ctttgcttca aagggaaaaa ggtttcccac 541 agtccgttaa attaaattgc acccgactaa atcagagaaa caaacgccaa tcctgtacat 601 cccctccgat cccttttgga ccaactttaa taagaagtag gaatgcgtgt acatcttgca 661 attttccttg tggtcacatt gttgcggaaa ggaaccccga ccggtgaaaa cgtcaacgta 721 aacgagtatg gagttctgtg caagatatgg aggctccacc aagggcttga gttacctgac 781 ggcgagaaga ccaaagagga tggggtagca attgtgaaag aaatatcaga tttgtggata 841 gttacattgg ccaacgaatc cgaggaaaaa gattatggcg gaaaagtgga cagagagCCT 901 AGGtagggat aacagggtaa tgtacCTAGC TTTCCACCCA GCGCGGGTGC ATTCTGGCTC 961 TTATATATAC TTATTGTCAT GACAGAGTAT ATTGTACTGT GTTGATAAGG GACGGGTAAC 1021 TGTATTGAAG AGCCGATGCT TTTGACATGT TAGATATAAT ATGTTTTATT GTAAAGTCAA 1081 TACAACACAC AATAGGATAA TAATGATAAA GTTAAAAAAG TATATATAGT AATAGAAATA 1141 TATCTTATAT AGGAAAGATT AAGCAGTAAA AGTAGCGCTT ACGGCGTACG GTTCCCTATC 1201 AGTGATAGAG ATCTCCCTAT CAGTGATAGA GATCCCTGAG TACTGAGTTT AACATGTTCT 1261 CGTCccgggc tgcacgcgcc ttcgagtttt ttttcctttt ccccattttt ttcaacttga 1321 agacttcaat tacaccaaaa agtaaaattc acaagcttat gccaaagaag aagcgaaagg 1381 tacatatgaa aaacatcaaa aaaaaccagg taatgaacct gggtccgaac tctaaactgc 1441 tgaaagaata caaatcccag ctgatcgaac tgaacatcga acagttcgaa gcaggtatcg 1501 gtctgatcct gggtgatgct tacatccgtt ctcgtgatga aggtaaaacc tactgtatgc 1561 agttcgagtg gaaaaacaaa gcatacatgg accacgtatg tctgctgtac gatcagtggg 1621 tactgtcccc gccgcacaaa aaagaacgtg ttaaccacct gggtaacctg gtaatcacct 1681 ggggcgccca gactttcaaa caccaagcat tcaacaaact ggctaacctg ttcatcgtta 1741 acaacaaaaa aaccatcccg aacaacctgg ttgaaaacta cctgaccccg atgtctctgg 1801 catactggtt catggatgat ggtggtaaat gggattacaa caaaaactct accaacaaat 1861 cgatcgtact gaacacccag tctttcactt tcgaagaagt agaatacctg gttaagggtc 1921 tgcgtaacaa attccaactg aactgttacg taaaaatcaa caaaaacaaa ccgatcatct 1981 acatcgattc tatgtcttac ctgatcttct acaacctgat caaaccgtac ctgatcccgc 2041 agatgatgta caaactgccg aacactatct cctccgaaac tttcctgaaa tacccatacg 2101 atgttccaga ttacgcttgc taatagGATC CTGCCCATTT AGTTGGCTTT TCCCTTGTCT 2161 CGTGTCTTTT CCGTGGAAAG GTTCCCGGAG TAATCTGATG GCACAGCAGG GAGGTGCGCC 2221 TGCAGGTTGG TTAGGAAGGG GGGATGATGT AAAAGAAGAA AATGGGGGGA TATCTTATGT 2281 TTAAGAGGAT AAATAATGTG AAGGGGCTTT ATATGCTTGC TTGGTTGTCT CGTTGGTGCA 2341 TGGGGAATCT GCATGTTTGC TTTGGAGCAC GCGTGGTACA TCAATGGGAT CATATCTTGC 2401 TGCCTCCCGC AGTCACCGTG CGGAGCTGCC GGTGCCCCGT TTCTCATAGG CGTGTGCACC 2461 TTCCGTCTGA GACCTGTAAA TGGTTAAAAA GGAATATATA ATTACCTTTT GAAAAGTGGT 2521 AAAAAACGAA TATATGTTTT TTTTTGGTTT TAATACGCTC TTTTGTGTGT ATGAGAGGAA 2581 TAAATAAGTG TGTGTGTGTG TGTGTTGTTG TTGTTGAAAA AATGAAGGAC ACGAAAGCGC 2641 TCATTAGGGG TGGGGATACA CCAGGCATAA TTATCCGAAA ATATTTTGGT GCACCAAATA 2701 AGTGAACATA CTGACGAAAT CAAAGAGTTG GAGGATGGAT AGGGAGGCCT CATTGGCAGT 2761 GGTAAATTGA TTTGACTTGA ATATGATACC CTCTTACTGT TTCATTGCCA CTACAAGTTG 2821 GTTTCCTTCC CCTGCAGGCA TGCTGGAAAG CTAGGTACCT GATTAATACG ACTCACTATA 2881 GGGAGATCTC CCGTACCGGG GGCACAGCAA GGTCTTCTGA AATTCATGTT TTTTTTTTTT 2941 TACTCTGCAT TGCAGTCTCC GCTCTTATTA GTTTTGCTTT ACGTAAGGTC TCGTTGCTGC 3001 CATAAAATAA GCTCTAGAAC TAGTGATGAA AAAGCCTGAA CTCACCGCGA CGTCTGTCGA 3061 GAAGTTTCTG ATCGAAAAGT TCGACAGCGT CTCCGACCTG ATGCAGCTCT CGGAGGGCGA 3121 AGAATCTCGT GCTTTCAGCT TCGATGTAGG AGGGCGTGGA TATGTCCTGC GGGTAAATAG 3181 CTGCGCCGAT GGTTTCTACA AAGATCGTTA TGTTTATCGG CACTTTGCAT CGGCCGCGCT 3241 CCCGATTCCG GAAGTGCTTG ACATTGGGGA ATTCAGCGAG AGCCTGACCT ATTGCATCTC 3301 CCGCCGTGCA CAGGGTGTCA CGTTGCAAGA CCTGCCTGAA ACCGAACTGC CCGCTGTTCT 3361 GCAGCCGGTC GCGGAGGCCA TGGATGCGAT CGCTGCGGCC GATCTTAGCC AGACGAGCGG 3421 GTTCGGCCCA TTCGGACCGC AAGGAATCGG TCAATACACT ACATGGCGTG ATTTCATATG 3481 CGCGATTGCT GATCCCCATG TGTATCACTG GCAAACTGTG ATGGACGACA CCGTCAGTGC 3541 GTCCGTCGCG CAGGCTCTCG ATGAGCTGAT GCTTTGGGCC GAGGACTGCC CCGAAGTCCG 3601 GCACCTCGTG CACGCGGATT TCGGCTCCAA CAATGTCCTG ACGGACAATG GCCGCATAAC 3661 AGCGGTCATT GACTGGAGCG AGGCGATGTT CGGGGATTCC CAATACGAGG TCGCCAACAT 3721 CTTCTTCTGG AGGCCGTGGT TGGCTTGTAT GGAGCAGCAG ACGCGCTACT TCGAGCGGAG 3781 GCATCCGGAG CTTGCAGGAT CGCCGCGGCT CCGGGCGTAT ATGCTCCGCA TTGGTCTTGA 3841 CCAACTCTAT CAGAGCTTGG TTGACGGCAA TTTCGATGAT GCAGCTTGGG CGCAGGGTCG 3901 ATGCGACGCA ATCGTCCGAT CCGGAGCCGG GACTGTCGGG CGTACACAAA TCGCCCGCAG 3961 AAGCGCGGCC GTCTGGACCG ATGGCTGTGT AGAAGTACTC GCCGATAGTG GAAACCGACG 4021 CCCCAGCACT CGTCCGAGGG CAAAGGAATA GAGTAGATGC CGACCGGGAT cgatccccCG 4081 ATCCTAACAC CGGGTTGTGT GGCCAAAATT GTTCTGTAGT CGCTGTGAGT TGACACGGCT 4141 AGTGCTTATG ATTTTCCTCG CGTGTGGTGC CTGTACTCAG CCCTATGCCT TATTTGCAAC 4201 ACATTTACGT ACAGCGCACA AGAGAAGAGA AGATCACTTG AAGATAATAA ATATAGGGTT 4261 GTAGGCATCT TGTTTAACTC AAATTTTCTC GTCTTGGTGT GTCGACATGA TTGAAATAGT 4321 GCCACCAGTT GTGTTTGATG CGTTTGTTAT CTATGCAGTA TTCTGCAAGG CCTCGGAGAT 4381 CCTAACACCG GGTTGTGTGG CCAAAATTGT TCTGTAGTCG CTGTGAGTTG ACACGGCTAG 4441 TGCTTATGAT TTTCCTCGCG TGTGGTGCCT GTACTCAGCC CTATGCCTTA TTTGCAACAC 4501 ATTTACGTAC AGCGCACAAG AGAAGAGAAG ATCACTTGAA GATAATAAAT ATAGGGTTGT 4561 AGGCATCTTG TTTAACTCAA ATTTTCTCGT CTTGGTGTGT CGACATGATT GAAATAGTGC 4621 CACCAGTTGT GTTTGATGCG TTTGTTATCT ATGCAGTATT CTGCAGGCTG CTAACAAAGC 4681 CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG 4741 GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCTGAAAGGA GAACTATATC CGGATAGGGC 4801 TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC 4861 ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG GAGAACTATA 4921 TCCGGATagg gcCCaaaaga cgcagcggtc ttgggaaacg aagccgcaaa aggtgggaca 4981 atagtcaaaa attgcggtgg aaccggggaa aacgaggcca acggcggaaa tgtgggtatt 5041 tctttagcga acgacatcct gtgtctctgc tacctgcgtg gaagcggcta cgcttccgat 5101 cagaaaatgt gcttggacgg cgtgactggt ggatccaaca ataacgcctt aaattctgcc 5161 ctcagcgact taaccaatga ctggccgaaa ttaaagaatg gatgccccaa gcatcaagga 5221 aagacgacca ccgcctccat cgaggcttcc ctggccgctt tccatgcaaa gctcggagcc 5281 caggcgaaca aggctgacgc cagccaccaa acagggaggt acatattggg cattgcgacg 5341 acgccaaata caggctgtaa cggggcccag aaccaatttt gcgtcaacta caaacgccag 5401 ctagcaggca ccgacaaagg gatcccgtgg gagcgcaagg tccggggagc cgaagataag 5461 ctcgtggagg cggacactat gacgcgaatg gtagaaatgc taattcatcg gctggagaac 5521 ttgaactgga cagcttgggg aatatacgaa gacgcgctgg aacatgtaga tgcccatgaa 5581 tcaggacgta tcgagggcgg ctccggcacc aatgccgctg ccagcaccta tccaaaagaa 5641 aaacaacaaa atgcaatttc aaacgagagc ccagtatcta cttcgggata cgtaggagtg 5701 ccactaggtt ggttaatatc ggcttcactc ttccctGcgg ccgctcgaga attcGATGGC 5761 TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC CCTTTAGGGT TCCGATTTAG TGCTTTACGG 5821 CACCTCGACC CCAAAAAACT TGATTAGGGT GATGGTTCAC GTAGTGGGCC ATCGCCCTGA 5881 TAGACGGTTT TTCGCCCTTT GACGTTGGAG TCCACGTTCT TTAATAGTGG ACTCTTGTTC 5941 CAAACTGGAA CAACACTCAA CCCTATCTCG GTCTATTCTT TTGATTTATA AGGGATTTTG 6001 CCGATTTCGG CCTATTGGTT AAAAAATGAG CTGATTTAAC AAAAATTTAA CGCGAATTTT 6061 AACAAAATAT TAACGTTTAC AATTTCCCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA 6121 CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC 6181 CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG 6241 TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC 6301 TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG 6361 ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA 6421 GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TATTGACGCC GGGCAAGAGC 6481 AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG 6541 AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA 6601 GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG 6661 CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA 6721 ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGTAGCAATG GCAACAACGT 6781 TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT 6841 GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT 6901 TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG 6961 GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA 7021 TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC 7081 TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA 7141 AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT 7201 TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT 7261 TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT 7321 GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC 7381 AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG 7441 TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG 7501 ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT 7561 CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC 7621 TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG 7681 ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG 7741 GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT 7801 TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT 7861 TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG 7921 ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA 7981 CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCCAATA CGCAAACCGC 8041 CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA CGACAGGTTT CCCGACTGGA 8101 AAGCGGGCAG TGAGCGCAAC GCAATTAATG TGAGTTAGCT CACTCATTAG GCACCCCAGG 8161 CTTTACACTT TATGCTTCCG GCTCGTATGT TGTGTG //